Advisor

Hyslop, Richard M.

Committee Member

Pacheco, Kimberly A.O.

Committee Member

Pringle, David L.

Department

Chemistry and Biochemistry

Institution

University of Northern Colorado

Type of Resources

Text

Place of Publication

Greeley (Colo.)

Publisher

University of Northern Colorado

Date Created

8-1-2014

Genre

thesis

Extent

65 pages

Digital Origin

Born digital

Abstract

Fatty acids are carboxylic acids with long alkane or alkene chains and are important components of living organisms. The presence of the carboxylic acid is analytically very important since it allows for derivatization with fluorescence reagents such as 9-chloromethyl-anthracene (9-CMA) for HPLC analysis. A method for the analysis of fatty acids using 9-CMA and 2-bromomethyl-anthraquinone (MAQ-Br) was developed. The method utilizes a modified protocol previously used for the analysis of short-chain carboxylic acids with 9-CMA. The modified protocol was applied to medium- and long-chain fatty acids for analysis by HPLC with UV-visible and fluorescence detection. After successful derivatization and analysis using 9-CMA, MAQ-Br was used for derivatization with successful results. Six fatty acid standards were derivatized with 9-CMA and utilized to develop a separation method and quantification. With the use of a Zorbax Rx C8 column, good separation was achieved and calibration curves ranging from 1 to 100 pmol with good linearity and R2 values. The limit of detection was approximately 50 fmol and the maximum limit of quantitation was approximately 100 pmol for the 9-CMA derivatives. After successful separation and quantitation, analyses of fat-free cooking spray and cloprostenol, a prostaglandin, were performed.

Degree type

MS

Degree Name

Master

Language

English

Local Identifiers

TapiaThesis2014

Rights Statement

Copyright is held by author.

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